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International Journal ofMolecular Sciences ArticleA SubClone of RAW264.7Cells Form OsteoclastLike Cells Capable of Bone Resorption Faster than Parental RAW264.7 through Increased De Novo Expression
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How to fill out a sub-clone of raw2647-cells
How to fill out a sub-clone of raw2647-cells
01
Thaw the frozen sub-clone of RAW264.7 cells by quickly warming the vial in a 37°C water bath.
02
Once the cells have completely thawed, transfer the contents of the vial into a sterile centrifuge tube.
03
Add an appropriate volume of complete growth medium to the centrifuge tube containing the cells.
04
Gently mix the cells and medium by pipetting up and down several times.
05
Centrifuge the cell suspension at low speed (e.g., 200 x g) for 5 minutes to pellet the cells.
06
Carefully remove the supernatant without disturbing the cell pellet.
07
Resuspend the cell pellet in fresh complete growth medium.
08
Count the cells using a hemocytometer or an automated cell counter.
09
Adjust the cell concentration to the desired density by diluting with complete growth medium.
10
Plate the cells into appropriate culture vessels or plates.
11
Incubate the cells in a humidified incubator at 37°C with 5% CO2.
12
Monitor the cells regularly under a microscope to assess their growth and confluency.
Who needs a sub-clone of raw2647-cells?
01
Researchers working in the field of immunology or cancer biology may need a sub-clone of RAW264.7 cells.
02
These cells are commonly used as a model system for studying macrophage activation, phagocytosis, and inflammation.
03
They can be used to investigate various aspects of immune responses, cytokine production, and cellular signaling pathways.
04
Pharmaceutical companies may also use RAW264.7 cells for drug screening and development purposes.
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What is a sub-clone of raw2647-cells?
A sub-clone of RAW264.7 cells refers to a distinct line of cells derived from the original RAW264.7 cell line, selected for specific characteristics or traits.
Who is required to file a sub-clone of raw2647-cells?
Researchers and laboratories that use or share sub-clones of RAW264.7 cells for research purposes are typically required to file appropriate documentation.
How to fill out a sub-clone of raw2647-cells?
Filling out a sub-clone of RAW264.7 cells generally involves providing relevant data, such as the cell line origin, characteristics, and purpose of use, often in a standardized form.
What is the purpose of a sub-clone of raw2647-cells?
The purpose of creating a sub-clone of RAW264.7 cells is to isolate and study specific cellular behaviors, genetic traits, or responses to treatments under controlled laboratory conditions.
What information must be reported on a sub-clone of raw2647-cells?
Information to be reported on a sub-clone of RAW264.7 cells usually includes the cell line designation, parent cell line, genetic modifications, and experimental applications.
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