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240 County Road
Ipswich, MA 019382723Tel 9789275054
Fax 9789211350www.neb.com
info@neb.comNew England Biol abs
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How to fill out random primer 9
How to fill out random primer 9
01
Prepare the reaction mix: In a sterile microcentrifuge tube, combine the following components:
02
- DNA template: Add the desired amount of DNA template that needs to be amplified.
03
- Random primer 9: Add the appropriate volume of random primer 9. This can vary depending on the protocol and the desired final concentration of the primer.
04
- Nuclease-free water: Add enough nuclease-free water to bring the total reaction volume to the desired final volume.
05
Mix the components well by gently pipetting up and down.
06
Incubate the reaction mix at a suitable temperature for the primers to anneal to the DNA template. This temperature can vary depending on the specific primer sequence and the melting temperature of the DNA template.
07
Once the primers have annealed, the reaction mix can be used for downstream applications such as PCR amplification, DNA sequencing, etc.
Who needs random primer 9?
01
Random primer 9 is commonly used in molecular biology research and techniques such as
02
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- Primer extension assays
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It is required by scientists, researchers, and technicians who work in these areas and need to amplify specific DNA sequences using random primers.
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