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Proteomics 2009, 9, 671685671DOI 10.1002/pmic.200800441RESEARCH ARTICLEProteomic profiling of antisenseinduced exon skipping reveals reversal of pathobiochemical abnormalities in dystrophic mdx diaphragm

How to fill out proteomic profiling of antisense-induced

How to fill out proteomic profiling of antisense-induced

1. Start by selecting the appropriate antisense molecules and designing the experimental setup.
2. Perform the necessary cell culture or animal model experiments to induce antisense expression.
3. Collect the samples of interest, such as cells or tissues, at the desired time points after inducing antisense expression.
4. Extract the proteins from the collected samples using suitable protein extraction techniques.
5. Quantify the extracted proteins using methods like Bradford assay or BCA assay.
6. Prepare the proteins for proteomic profiling by cutting them into smaller peptides using enzymes like trypsin.
7. Use mass spectrometry techniques, such as liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), to identify and quantify the peptides.
8. Analyze the obtained data using appropriate bioinformatics tools to identify differentially expressed proteins and perform functional annotations.
9. Validate the identified proteins using techniques like Western blotting or immunohistochemistry.
10. Interpret the results and draw conclusions about the impact of antisense-induced changes on the proteome.

Who needs proteomic profiling of antisense-induced?

1. Proteomic profiling of antisense-induced is needed by researchers and scientists studying the effects of antisense molecules on protein expression.
2. Pharmaceutical companies developing antisense-based therapies can benefit from proteomic profiling to understand the molecular changes induced by their molecules.
3. Biomedical researchers investigating the roles of specific proteins in disease processes can utilize proteomic profiling of antisense-induced to identify potential therapeutic targets.
4. Academic institutions and research laboratories focused on proteomics and gene regulation can utilize this technique to further advance their understanding of antisense-induced proteomic changes.

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What is proteomic profiling of antisense-induced?

Proteomic profiling of antisense-induced refers to the systematic analysis of the protein expression profiles that result from the modulation of gene expression through antisense oligonucleotides, which are designed to bind to specific messenger RNA (mRNA) and inhibit gene translation.

Who is required to file proteomic profiling of antisense-induced?

Research institutions, biotechnology companies, and pharmaceutical firms conducting studies involving antisense oligonucleotides and their effects on protein expression are typically required to file proteomic profiling of antisense-induced.

How to fill out proteomic profiling of antisense-induced?

Filling out the proteomic profiling of antisense-induced involves collecting data on protein expression levels, validating the findings through appropriate assays, and detailing the methodologies used in the study, followed by submitting the information to relevant regulatory bodies or institutional review boards as required.

What is the purpose of proteomic profiling of antisense-induced?

The purpose of proteomic profiling of antisense-induced is to understand the biological effects of antisense oligonucleotides on protein expression, which can help in evaluating their therapeutic potential and safety in various disease models.

What information must be reported on proteomic profiling of antisense-induced?

Reported information should include protein expression levels, comparison to control groups, methodologies, specific antisense oligonucleotide sequences used, any observed biological effects, and statistical analyses of the data.