Get the free Quick Ligation Kit
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Ipswich, MA 019382723Tel 9789275054
Fax 9789211350www.neb.com
info@neb.comNew England Biol abs
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How to fill out quick ligation kit
How to fill out quick ligation kit
01
Thaw the quick ligation kit on ice.
02
Set up a reaction mixture by combining the following components in a microcentrifuge tube:
03
- 1 μl of DNA fragment to be ligated
04
- 1 μl of 10X ligation buffer
05
- 1 μl of quick ligase
06
- 7 μl of nuclease-free water
07
Mix the reaction mixture well by gently pipetting up and down.
08
Incubate the reaction mixture at 25-30°C for 5-15 minutes.
09
Heat inactivate the quick ligase by incubating the reaction mixture at 65°C for 10 minutes.
10
Proceed with downstream applications or store the ligated DNA at -20°C until further use.
Who needs quick ligation kit?
01
Molecular biologists involved in DNA cloning procedures.
02
Researchers who need to join DNA fragments together in a quick and efficient manner.
03
Scientists working on genetic engineering or genetic modification studies.
04
Laboratories working on recombinant DNA technology and gene editing techniques.
05
Anyone requiring a fast and reliable method for DNA ligation.
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What is quick ligation kit?
The quick ligation kit is a tool used in molecular biology to facilitate the process of joining DNA fragments together, particularly for cloning purposes.
Who is required to file quick ligation kit?
Researchers and laboratories that need to perform DNA ligation for cloning and genetic engineering experiments are required to use the quick ligation kit.
How to fill out quick ligation kit?
To use the quick ligation kit, one must prepare the desired DNA fragments, mix them with the ligation buffer and ligase enzyme provided in the kit, and incubate under the recommended conditions.
What is the purpose of quick ligation kit?
The purpose of the quick ligation kit is to enable rapid and efficient ligation of DNA fragments, which is essential for cloning and constructing recombinant DNA.
What information must be reported on quick ligation kit?
Typically, the information to be reported includes the types and concentrations of DNA fragments used, the ligation conditions, and the results of the ligation process.
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