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Supplementary Table 1. Labelfree MS identified 516 proteins from the mitochondrial fractions of Pink1 and WT D. melanogasterIdentifier Protein nameQ94523 Succinate dehydrogenase [ubiquinone] flavoprotein
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01
Start by preparing a protein solution containing the desired protein that will mediate the assembly of succinate.
02
In a separate container, prepare a succinate solution with the appropriate concentration.
03
Mix the protein solution and the succinate solution together gently, ensuring that the two solutions are thoroughly combined.
04
Allow the protein-mediated assembly of succinate to occur by incubating the mixture at the appropriate temperature and for the necessary amount of time.
05
Once the assembly is complete, carefully separate the assembled succinate from any unreacted components or by-products.
06
Purify the assembled succinate as necessary to remove any impurities or contaminants.
07
The protein-mediated assembly of succinate is now complete and can be used for further experiments or applications.

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01
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Protein-mediated assembly of succinate refers to the biological process where specific proteins facilitate the organization and formation of succinate molecules within metabolic pathways.
Researchers or organizations conducting studies that involve protein-mediated assembly of succinate are typically required to file relevant documentation.
To fill out protein-mediated assembly of succinate, one must provide detailed data on the proteins involved, experimental conditions, and outcomes of the assembly processes being studied.
The purpose is to understand and document the role of proteins in the assembly of succinate, which is crucial for various biochemical processes and pathways.
Information on protein types, concentrations, assembly conditions, results, and any anomalies observed during the process must be reported.
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