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Test Definition: CATP Catecholamine Fractionation, Free, Plasma___ OverviewUseful For Diagnosing pheochromocytoma and paraganglioma, as an auxiliary test to fractionated plasma and urine metanephrine
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How to fill out catecholamine fractionation plasma
How to fill out catecholamine fractionation plasma
01
Start by collecting a plasma sample from the patient.
02
Centrifuge the plasma sample at high speed to separate it from the cellular components.
03
Transfer the supernatant to a new tube, being careful not to disturb the pellet.
04
Add a suitable solvent to the plasma sample in order to extract the catecholamines.
05
Mix the sample thoroughly to ensure proper extraction of the catecholamines.
06
Centrifuge the sample once again to separate the solvent phase from the plasma phase.
07
Transfer the solvent phase to a new tube and evaporate it under reduced pressure to remove the solvent.
08
Reconstitute the residue in a suitable buffer or solvent for analysis.
09
Finally, analyze the catecholamine content in the fractionated plasma using a suitable method, such as high-performance liquid chromatography (HPLC) or enzyme immunoassay.
Who needs catecholamine fractionation plasma?
01
Catecholamine fractionation plasma is needed for various medical and research purposes.
02
Some individuals who may benefit from catecholamine fractionation plasma are those with suspected adrenal tumors,
03
pheochromocytomas, neuroblastomas, or other conditions involving the abnormal production or regulation of catecholamines.
04
It is also useful for monitoring catecholamine levels in patients undergoing treatment for such conditions or for research studies investigating catecholamine metabolism and its role in different diseases.
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