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WASHINGTON STATE PATROL CODES LABORATORY STR ANALYSIS PROCEDURES CRIME LABORATORY DIVISION January 2024Washington State Patrol Crime Laboratory Division CODES Lab STR Procedures Manageable of Contents INTRODUCTION

How to fill out codis laboratory str analysis

How to fill out codis laboratory str analysis

1. Gather all the necessary equipment for conducting the CODIS laboratory STR analysis, including sample collection kits, DNA extraction kits, PCR reagents, and electrophoresis equipment.
2. Begin by collecting DNA samples from the desired individuals or sources using the provided collection kits. Follow the specified procedures for sample collection to ensure accurate results.
3. Extract the DNA from the collected samples using the DNA extraction kits. Follow the manufacturer's instructions to isolate the DNA from other cellular components.
4. Set up the PCR reaction by combining the extracted DNA, PCR reagents, and primers specific to the STR markers of interest. Follow the recommended PCR conditions for optimal amplification.
5. Run the PCR reaction using a thermal cycler. The PCR amplification will produce many copies of the targeted STR markers in the DNA samples.
6. Analyze the PCR products using gel electrophoresis. Load the PCR products onto an agarose gel and apply an electric current to separate the fragments based on their sizes.
7. Stain the gel with a suitable DNA dye and visualize the separated DNA fragments using UV light or an imaging system. The different alleles at each STR marker will appear as distinct bands on the gel.
8. Measure the sizes and intensities of the bands using appropriate software or by comparing them to DNA size standards. Record the data for each of the analyzed STR markers.
9. Analyze the obtained data based on the specific STR analysis objective, such as determining the genetic relationship between individuals or comparing DNA evidence from a crime scene.
10. Interpret the results by comparing the obtained DNA profiles with known reference samples or databases. This analysis can help identify individuals, establish paternity/maternity, or provide forensic evidence.

Who needs codis laboratory str analysis?

1. CODIS laboratory STR analysis is typically needed by forensic scientists and law enforcement agencies involved in criminal investigations.
2. It is also utilized in paternity/maternity testing to establish biological relationships between individuals.
3. Other fields that may require CODIS laboratory STR analysis include genetic research, population genetics, immigration case evaluations, and disaster victim identifications.

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What is codis laboratory str analysis?

CODIS (Combined DNA Index System) laboratory STR (Short Tandem Repeat) analysis refers to the process of analyzing specific regions of DNA that are made up of repeated sequences. This analysis is used for identifying individuals based on their DNA profiles and is primarily utilized in forensic science and criminal investigations.

Who is required to file codis laboratory str analysis?

Law enforcement agencies and forensic laboratories that collect and analyze DNA samples for criminal investigations and legal evidence are required to file CODIS laboratory STR analysis.

How to fill out codis laboratory str analysis?

Filling out CODIS laboratory STR analysis typically involves entering DNA profile data into a standardized format, including information about the sample collection, analysis methods used, and the resulting DNA profile. Specific details should adhere to the guidelines provided by the CODIS system and relevant authorities.

What is the purpose of codis laboratory str analysis?

The purpose of CODIS laboratory STR analysis is to establish a complete DNA profile from biological samples, which can be matched against profiles in the CODIS database to identify suspects, victims, or missing persons, and aid in solving criminal cases.

What information must be reported on codis laboratory str analysis?

The information that must be reported includes the DNA profile data, the source of the biological sample, the date of collection, the methods used for analysis, and any pertinent case identifiers.