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Indirect, Amino Ally Labelling of CDA MicroarraysSlide # ______ Cy3 Cy5 Sample being labelled total amount in UG (3050 ideal)CDA synthesis in NG/UL after RT(ideally 1530 ng/UL in 60 UL volume)Code
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01
Prepare the amino allyl-modified nucleotide mixture according to the protocol.
02
Denature the target DNA or RNA by heating at 70-95°C for a few minutes.
03
Mix the denatured DNA or RNA with the amino allyl-modified nucleotide mixture and incubate at a specific temperature.
04
Stop the reaction by adding the termination solution.
05
Purify the labeled DNA or RNA using purification columns or other methods.
06
Measure the concentration and quality of the labeled product using spectrophotometry or other techniques.

Who needs amino allyl incorporation method?

01
Researchers working in the field of molecular biology
02
Scientists studying gene expression
03
Those interested in labeling nucleic acids for further analysis or manipulation

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The amino allyl incorporation method is a chemical technique used in molecular biology for incorporating amino allyl groups into nucleic acids, which allows for subsequent labeling and analysis.
Researchers and laboratories involved in nucleotide labeling or that utilize the amino allyl incorporation method in their experiments are typically required to file documentation related to this method.
Filling out the amino allyl incorporation method generally involves providing detailed information about the reagents used, procedure followed, and the results obtained during the experimental process.
The purpose of the amino allyl incorporation method is to enable the labeling of nucleic acids for visualization or quantification in various molecular biology applications such as microarray analysis or PCR.
Information that must be reported includes the specific reagents used, concentrations, experimental conditions, duration, and any calibration standards employed during the process.
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