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Boris preprint DOI: https://doi.org/10.1101/2023.05.24.541142; this version posted May 24, 2023. The copyright holder for this preprint (which was not certified by peer review) is the author/funder,

How to fill out multi-modal cryo-em reveals trimers

How to fill out multi-modal cryo-em reveals trimers

1. Thaw the multi-modal cryo-EM grid and check for any ice contamination
2. Apply a thin layer of fiducial markers on the grid
3. Load the grid into the cryo-EM instrument and align it properly
4. Collect images of the trimers at multiple angles and exposures
5. Use software to analyze the images and reconstruct the 3D structure of the trimers

Who needs multi-modal cryo-em reveals trimers?

1. Researchers studying the structure of protein complexes
2. Scientists investigating the function of viral spikes
3. Biologists studying membrane proteins

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What is multi-modal cryo-em reveals trimers?

Multi-modal cryo-electron microscopy (cryo-EM) reveals trimers by using advanced imaging techniques to visualize the three subunit structures of macromolecules at high resolution, allowing researchers to study their functionalities and interactions.

Who is required to file multi-modal cryo-em reveals trimers?

Researchers and institutions conducting cryo-EM studies that focus on trimers within their scientific research are typically required to file results and data associated with their findings.

How to fill out multi-modal cryo-em reveals trimers?

To fill out multi-modal cryo-EM reveals trimers forms, researchers need to provide detailed information about the experimental design, methods used, results obtained, and data analysis in accordance with the specific guidelines set forth by the relevant governing body or journal.

What is the purpose of multi-modal cryo-em reveals trimers?

The purpose of multi-modal cryo-EM reveals trimers is to obtain high-resolution structural information and functional insights about trimeric proteins and complexes, which can aid in understanding biological processes and developing therapeutic approaches.

What information must be reported on multi-modal cryo-em reveals trimers?

Information that must be reported includes the methodology, experimental conditions, data quality metrics, structural findings, and any related biological implications or applications.