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QUOTATION FORMFOR RECOMBINANT PROTEIN PRODUCTION IN MAMMALIAN CELLS Request Number: Status: Please fill out the following required information completely Name (first/last): Position or Title:Company/Institute:
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How to fill out protein expression amp purification

01
Obtain the protein of interest and design an expression construct for it.
02
Transform the expression construct into a suitable host organism.
03
Grow the host organism in a suitable growth medium and induce protein expression.
04
Harvest the cells and break them open to release the proteins.
05
Purify the protein using different techniques such as chromatography.
06
Verify the purity and integrity of the protein.

Who needs protein expression amp purification?

01
Researchers in the fields of biochemistry, molecular biology, and biotechnology who are studying protein structure and function.
02
Pharmaceutical companies looking to produce recombinant proteins for therapeutic use.
03
Biotech companies working on the development of new drugs or biologics.

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Protein expression and purification is the process of producing proteins in a host organism and subsequently isolating and purifying them for research or therapeutic use.
Researchers, scientists, or organizations that conduct studies or trials involving the expression and purification of proteins are required to file related documentation.
Filling out protein expression and purification documentation typically involves providing details such as the host organism used, the methods of expression and purification, and any relevant experimental conditions.
The purpose of protein expression and purification is to obtain large quantities of a specific protein in a pure form for use in research, drug development, or clinical applications.
Information that must be reported includes the type of protein being expressed, the methods used for expression and purification, any modifications made to the protein, and results from characterization studies.
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