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CryoEM movie data processing protocol using RELION, CTFFIND4 or GCTF, and MotionCor2 (and possibly CryoSPARC) Written 4/1/2017, updated 8/25/17, 9/20/17, 7/17/19 I suggest using the latest versions

How to fill out real-time cryo-em data pre-processing

How to fill out real-time cryo-em data pre-processing

1. Start by importing the raw data files into the pre-processing software.
2. Check and adjust the alignment of the images to ensure they are properly aligned.
3. Apply normalization techniques to correct for any variability in the data.
4. Remove any background noise or artifacts from the images.
5. Perform particle picking to identify and extract individual particles from the images.
6. Classify the particles based on their shapes and sizes to further analyze the data.

Who needs real-time cryo-em data pre-processing?

1. Researchers and scientists working in the field of structural biology who are studying the three-dimensional structures of biological molecules.
2. Biochemists and biophysicists who need to process large amounts of cryo-electron microscopy data quickly and efficiently.
3. Drug developers and pharmaceutical companies using cryo-em for drug discovery and protein structure determination.

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What is real-time cryo-em data pre-processing?

Real-time cryo-EM data pre-processing refers to the immediate management and refinement of cryo-electron microscopy data to enhance quality, ensure accuracy, and prepare it for further analysis. This process can involve correcting motion, improving contrast, and filtering noise from the collected image data.

Who is required to file real-time cryo-em data pre-processing?

Researchers and institutions that conduct cryo-electron microscopy studies and wish to submit their findings for publication or regulatory review are required to file real-time cryo-EM data pre-processing.

How to fill out real-time cryo-em data pre-processing?

To fill out real-time cryo-EM data pre-processing, users must gather relevant data on their cryo-EM experiments and results, follow any specified guidelines for reporting formats, and submit through the designated channels, ensuring all required fields are completed accurately.

What is the purpose of real-time cryo-em data pre-processing?

The purpose of real-time cryo-EM data pre-processing is to ensure that the collected data is of high quality, reliable, and suitable for subsequent analysis and interpretation, facilitating accurate scientific conclusions.

What information must be reported on real-time cryo-em data pre-processing?

Information that must be reported includes experimental conditions, instrument settings, data collection parameters, any corrections applied during processing, and details regarding data viability and reproducibility.