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ZANA Ilan DFASP2 Anne 20182019Matre de stage : Jean CHARLETLIMICS, Inserm U11 421 Table des matires 1Table des matires ..............................................................................................................
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How to fill out 53bp1mediated recruitment of rassf1a
How to fill out 53bp1mediated recruitment of rassf1a
01
Start by preparing the necessary materials including cell lines expressing GFP or RFP-tagged proteins, antibodies against 53bp1 and Rassf1a, and appropriate controls.
02
Seed the cells in a suitable culture dish and allow them to adhere and grow to the desired confluency.
03
Treat the cells as required to induce the recruitment of 53bp1 to DNA damage sites.
04
Fix the cells with paraformaldehyde and permeabilize them with Triton X-100.
05
Block the cells with a suitable blocking agent and incubate them with the primary antibodies against 53bp1 and Rassf1a.
06
Wash the cells and then incubate them with the secondary antibodies conjugated to fluorescent dyes.
07
Finally, visualize and analyze the recruitment of Rassf1a by 53bp1 using fluorescence microscopy or other imaging techniques.
Who needs 53bp1mediated recruitment of rassf1a?
01
Researchers studying DNA damage response pathways and protein-protein interactions in the context of cancer or other diseases may need to investigate 53bp1-mediated recruitment of Rassf1a.
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What is 53bp1mediated recruitment of rassf1a?
53bp1-mediated recruitment of Rassf1a refers to the process through which the protein 53BP1 promotes the binding of Rassf1a to specific sites within the cell, facilitating various cellular responses, particularly in DNA damage repair and cell cycle regulation.
Who is required to file 53bp1mediated recruitment of rassf1a?
The filing of documents or reports related to 53bp1-mediated recruitment of Rassf1a is typically required by researchers and professionals involved in molecular biology or genetic studies, particularly those studying DNA repair mechanisms.
How to fill out 53bp1mediated recruitment of rassf1a?
To fill out the relevant documentation for 53bp1-mediated recruitment of Rassf1a, ensure to provide accurate descriptions of experimental methods, data obtained, and any findings related to the interactions of these proteins.
What is the purpose of 53bp1mediated recruitment of rassf1a?
The purpose of 53bp1-mediated recruitment of Rassf1a is to enhance the cellular response to DNA damage, stabilize the genome, and prevent excessive cell division, thereby playing a critical role in tumor suppression.
What information must be reported on 53bp1mediated recruitment of rassf1a?
Information that must be reported includes the experimental conditions, quantitative data on recruitment efficacy, observation of cellular responses, and implications for DNA damage signaling pathways.
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