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EXPERIMENT 11 UV/VIS Spectroscopy and Spectrophotometry: Spectrophotometric Analysis of Potassium Permanganate Solutions. Outcomes After completing this experiment, the student should be able to:
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01
Prepare the UV-Vis spectrophotometer by turning it on and allowing it to warm up.
02
Set the wavelength range and resolution according to the requirements of the experiment.
03
Calibrate the instrument using a standard solution with known absorbance values.
04
Prepare the sample solutions to be analyzed, ensuring they are at room temperature and properly labeled.
05
Place the cuvette containing the sample solution in the spectrophotometer, making sure it is properly aligned.
06
Record the absorbance readings at specified wavelengths, following the instructions provided in the experiment protocol.
07
Repeat the measurements for each sample solution and calculate the average absorbance values.
08
Analyze the data obtained and draw conclusions based on the absorption spectra observed.

Who needs experiment 11 uvvis spectroscopy?

01
Students studying analytical chemistry or related fields who need to learn about UV-Vis spectroscopy techniques and applications.
02
Researchers working in laboratories who use UV-Vis spectroscopy for analysis of samples in various scientific disciplines.
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Experiment 11 UV-Vis spectroscopy involves the use of ultraviolet and visible light to analyze the absorbance and transmittance of samples, allowing for the determination of their concentration and molecular structure.
Individuals conducting Experiment 11 UV-Vis spectroscopy as part of a laboratory course or research study are typically required to file the results.
To fill out Experiment 11 UV-Vis spectroscopy, participants must record the wavelengths of light used, the absorbance values measured, any dilution factors, sample descriptions, and any relevant calculations for concentration.
The purpose of Experiment 11 UV-Vis spectroscopy is to quantify the concentration of analytes in a sample by measuring how much light is absorbed at specific wavelengths.
The information that must be reported includes sample identification, absorbance readings at specified wavelengths, calibration curves, and the calculated concentrations.
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