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Direct Quantitation of Five Immunosuppressant Drugs in VolumeControlled Dried Whole Blood Spots by a Fully Automated DSMLCMS System Richard J. Gibson, Jingshu Guo, Stephanie N. Samra, Thermo Fisher
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Prepare a standard solution of known concentration for the analyte of interest.
02
Measure the absorbance or fluorescence intensity of the standard solution with a spectrophotometer or fluorometer.
03
Create a calibration curve by plotting the measured absorbance or fluorescence intensity against the concentration of the standard solutions.
04
Measure the absorbance or fluorescence intensity of the unknown sample.
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Use the calibration curve to determine the concentration of the analyte in the unknown sample.

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Environmental scientists monitoring pollutant levels in air, water, or soil samples.
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Direct quantitation of five refers to a specific method of measuring and reporting the levels of five designated substances within a particular sample or context, adhering to regulatory guidelines.
Entities or individuals that possess, manage, or potentially discharge the five designated substances in regulated environments are required to file the direct quantitation of five.
To fill out the direct quantitation of five, individuals must gather relevant data about the substances, complete the designated forms accurately with the required metrics, and submit them to the appropriate regulatory body.
The purpose of direct quantitation of five is to ensure compliance with environmental regulations, monitor potential pollutants, and protect public health by providing accurate data on the presence of these substances.
The information that must be reported includes the identification of the substances, their measured quantities, the methods used for quantitation, and the time and location of sampling.
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