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PLOS ONE RESEARCH ARTICLEA universal RTqPCR assay for One Health detection of influenza A viruses Alexander Nagy ID1,2*, Lenka ernkova1, Kateina Kunteova1, Zuzana Dirbakova3, Saumya S. Thomas ID4,

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How to fill out a universal rt-qpcr assay

01

Begin by gathering all necessary equipment and reagents for the assay.

02

Thaw the RNA samples to be tested on ice and prepare the RT-qPCR master mix according to the manufacturer's instructions.

03

Add the RNA samples and master mix to the PCR plate in a sterile hood, being careful not to introduce any contaminants.

04

Run the RT-qPCR assay according to the specified thermal cycling conditions.

05

Analyze the results using appropriate software to determine the expression levels of the target genes.

Who needs a universal rt-qpcr assay?

01

Researchers studying gene expression levels across different samples or conditions may require a universal rt-qpcr assay to quantify RNA transcripts accurately.

02

Diagnostic laboratories may also use universal rt-qpcr assays to detect and quantify specific RNA targets in patient samples.

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What is a universal rt-qpcr assay?

A universal rt-qpcr assay is a laboratory technique used to quantify RNA by converting it to complementary DNA (cDNA) and amplifying specific target sequences, allowing for the detection and quantification of gene expression across various samples.

Who is required to file a universal rt-qpcr assay?

Researchers and laboratories that conduct studies involving RNA analysis, particularly in fields such as genomics, molecular biology, and diagnostics, are typically required to file a universal rt-qpcr assay.

How to fill out a universal rt-qpcr assay?

To fill out a universal rt-qpcr assay, researchers must provide details such as the sample information, assay conditions, target genes, reagents used, and any controls implemented during the experiment.

What is the purpose of a universal rt-qpcr assay?

The purpose of a universal rt-qpcr assay is to accurately measure RNA levels within a sample, which helps in understanding gene expression patterns, identifying biomarkers, and conducting various research studies.

What information must be reported on a universal rt-qpcr assay?

Information that must be reported on a universal rt-qpcr assay includes the sample identification details, list of primers and probes used, reaction conditions, data analysis methods, and results obtained from the assay.

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