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Datasheet for ABIN1884700Malondialdehyde ELISA Kit 1 Image10 Publications Go to Product pageOverview Quantity:96 testsTarget:Malondialdehyde (MDA)Reactivity:ChemicalMethod Type:Competition ELISADetection
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01
Equilibrate all reagents and samples to room temperature before starting the assay.
02
Prepare standard curve by diluting MDA standards provided in the kit with sample diluent.
03
Add samples, standards, and controls to the appropriate wells of the microplate.
04
Add the MDA conjugate to each well and incubate for the recommended time.
05
Wash the plate to remove unbound substances.
06
Add substrate solution to each well and incubate in the dark.
07
Stop the reaction by adding stop solution.
08
Read the optical density of each well at the specified wavelength.
09
Calculate the concentration of MDA in the samples using the standard curve.

Who needs malondialdehyde elisa kit?

01
Researchers studying oxidative stress and lipid peroxidation processes.
02
Clinicians monitoring patients with conditions related to oxidative damage such as atherosclerosis, diabetes, or neurodegenerative diseases.
03
Laboratories performing research on biomarkers of oxidative stress in various biological samples.
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The malondialdehyde ELISA kit is a laboratory tool used to measure the level of malondialdehyde (MDA) in biological samples, which is an indicator of oxidative stress and lipid peroxidation.
Researchers and laboratories conducting studies involving oxidative stress and its implications may be required to use and file results from a malondialdehyde ELISA kit.
To fill out a malondialdehyde ELISA kit, follow the kit instructions precisely, which typically includes preparing samples, adding reagents, incubating, and then reading the results using a plate reader.
The purpose of the malondialdehyde ELISA kit is to quantify the concentration of MDA in samples to assess the extent of oxidative stress and potential damage to cells.
Information typically reported includes sample concentrations of MDA, standard curves, assay details, experimental conditions, and any controls used during the analysis.
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