Get the free PKC zeta ELISA Kit - Product Details. PKC zeta ELISA Kit for Measurement of Mouse PK...

Datasheet for ABIN1116788PKC zeta ELISA Kit 1 Image Go to Product pageOverview Quantity:96 testsTarget:PKC zeta (PRKCZ)Reactivity:MouseMethod Type:Sandwich ELISADetection Range:0.15610 ng/mLMinimum

How to fill out pkc zeta elisa kit

How to fill out pkc zeta elisa kit

1. Remove the desired number of microtiter strips from the foil pouch and place them in a holder.
2. Add 100 µl of Standard, Control, or diluted samples into the appropriate wells.
3. Add 50 µl of HRP-Conjugate reagent to each well.
4. Cover the plate with a plate sealer and incubate for 1 hour at room temperature.
5. Aspirate the liquid from each well and wash four times with Wash Buffer.
6. Add 100 µl of Substrate Solution to each well and incubate for 30 minutes at room temperature in the dark.
7. Finally, add 100 µl of Stop Solution to each well and mix gently. The color will change from blue to yellow.
8. Read the absorbance at 450 nm within 30 minutes to determine the concentration of PKC zeta.

Who needs pkc zeta elisa kit?

1. Researchers studying protein kinase C zeta (PKC zeta) expression levels or activity.
2. Scientists investigating signal transduction pathways involving PKC zeta.
3. Laboratories conducting research on cancer, diabetes, inflammation, or neurodegenerative diseases where PKC zeta has been implicated.

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What is pkc zeta elisa kit?

The PKC Zeta ELISA Kit is a laboratory tool used to measure the levels of Protein Kinase C zeta (PKC zeta) in biological samples, typically for research purposes.

Who is required to file pkc zeta elisa kit?

Research laboratories and institutions conducting studies involving PKC zeta may be required to use the kit for data collection and analysis.

How to fill out pkc zeta elisa kit?

To use the PKC zeta ELISA Kit, follow the manufacturer's instructions, which typically include preparing samples, adding them to the plate, incubating, and then analyzing the results using a suitable detection method.

What is the purpose of pkc zeta elisa kit?

The purpose of the PKC Zeta ELISA Kit is to quantify PKC zeta levels in samples, which is important for understanding its role in various cellular processes and diseases.

What information must be reported on pkc zeta elisa kit?

Results typically reported include the concentration of PKC zeta in the samples, corresponding to the standards included in the assay, as well as any relevant experimental conditions.