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JURNAL FARMASI SAINS DAN KOMUNITAS, November 2020, 6975 pISSN 16935683; eISSN 25277146 doi: http://dx.doi.org/10.24071/jpsc.002362Vol. 17 No. 2ANTIBACTERIAL ACTIVITY AND TLCBIOAUTOGRAPHY ANALYSIS

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How to fill out antibacterial activity and tlc-bioautography

01

For antibacterial activity: Prepare culture media with bacterial strain, inoculate the media with the bacterial strain, incubate the culture, prepare the test sample, determine the antibacterial activity by measuring zone of inhibition.

02

For TLC-bioautography: Prepare thin layer chromatography plate, spot the sample on the plate, develop the plate in suitable solvent system, visualize the spots under UV light, place the plate on agar containing bacterial strain, incubate the plate, observe zones of inhibition.

Who needs antibacterial activity and tlc-bioautography?

01

Researchers in pharmaceutical industry to discover new antibiotics.

02

Scientists in academia studying natural products for potential antibacterial compounds.

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What is antibacterial activity and tlc-bioautography?

Antibacterial activity refers to the ability of a substance to inhibit the growth of bacteria. TLC-bioautography is a technique that combines thin-layer chromatography (TLC) with bioassays, allowing for the visualization of biological activity of compounds separated by TLC.

Who is required to file antibacterial activity and tlc-bioautography?

Entities involved in the research or development of antibacterial agents, such as pharmaceutical companies, researchers, and laboratories, are typically required to file antibacterial activity and tlc-bioautography results.

How to fill out antibacterial activity and tlc-bioautography?

To fill out antibacterial activity and tlc-bioautography, one must gather experimental data, present it in a structured format, document methodology, and ensure that results are reproducible. It is advised to follow specific guidelines provided by regulatory agencies.

What is the purpose of antibacterial activity and tlc-bioautography?

The purpose is to assess the effectiveness of compounds against bacteria, identify active components, and ensure safety and efficacy in medicinal products.

What information must be reported on antibacterial activity and tlc-bioautography?

The report should include details about the test organisms, concentrations used, methods of analysis, results of activity, and visual documentation of TLC bioautography.

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