Get the free QIAamp DSP Viral RNA Mini Kit Instructions for Use ( ...

DOCUMENT NO. ISSUE NO AMENDMENT NO ISSUE DATEMKAKB/BP/WS64 01 00 06.11.2016Worksheet MERSCOVRTPCR * RNA extraction using QIAamp Viral RNA Mini Kit (Qiagen) Lot No : ___ Test Performed By : ___ Expiry

How to fill out qiaamp dsp viral rna

How to fill out qiaamp dsp viral rna

1. Add 140 μL of sample to a 1.5 mL microcentrifuge tube.
2. Add 560 μL of Buffer AVL to the sample in the microcentrifuge tube.
3. Add 560 μL of ethanol (96-100%) to the sample in the microcentrifuge tube.
4. Mix the sample by vortexing for 15 seconds.
5. Load the sample onto a QIAamp Mini column placed in a 2 mL collection tube.
6. Centrifuge at 6,000 x g (8,000 rpm) for 1 minute.
7. Discard the flow-through and place the QIAamp Mini column back in the same 2 mL collection tube.
8. Add 500 μL of Buffer AW1 to the QIAamp Mini column.
9. Centrifuge at 6,000 x g (8,000 rpm) for 1 minute.
10. Discard the flow-through and place the QIAamp Mini column back in the same 2 mL collection tube.
11. Add 500 μL of Buffer AW2 to the QIAamp Mini column.
12. Centrifuge at maximum speed for 3 minutes to dry the membrane.
13. Place the QIAamp Mini column in a clean 1.5 mL microcentrifuge tube.
14. Add 60 μL of Buffer AVE directly onto the QIAamp membrane.
15. Incubate at room temperature for 1 minute and centrifuge at maximum speed for 1 minute to elute the RNA.

Who needs qiaamp dsp viral rna?

1. Laboratories conducting research on viral infections.
2. Healthcare facilities diagnosing viral diseases.
3. Biotechnology companies working on developing antiviral treatments.

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What is qiaamp dsp viral rna?

QIAamp DSP viral RNA is a reagent kit used for the isolation and purification of viral RNA from biological samples, facilitating the detection and analysis of viral infections.

Who is required to file qiaamp dsp viral rna?

Typically, laboratories and research institutions that conduct viral RNA testing or work with viral samples are required to utilize QIAamp DSP viral RNA for proper sample processing.

How to fill out qiaamp dsp viral rna?

Filling out QIAamp DSP viral RNA usually involves following the manufacturer's instructions for sample processing, which do not entail a formal 'filing' but rather a protocol for use.

What is the purpose of qiaamp dsp viral rna?

The purpose of QIAamp DSP viral RNA is to effectively extract and purify viral RNA to enable subsequent analysis, such as PCR testing for viral detection.

What information must be reported on qiaamp dsp viral rna?

Reported information generally includes sample identification, source of the sample, extraction date, and any relevant details about the viral RNA test performed.