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Protocol for Catalog # EK07109 Urotensin II (Rat) EIA Kit (range: 0100ng/ml)PHOENIX PHARMACEUTICALS, INC.INTRODUCTION This Enzyme Immunoassay kit is designed to detect a specific peptide and its related
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01
Thaw the assay plate and all reagents to room temperature.
02
Dilute the standards, controls, and samples as instructed in the protocol.
03
Add 50μl of standards, controls, and samples to the appropriate wells.
04
Add 50μl of biotinylated detection antibody to each well.
05
Incubate for 2 hours at room temperature on a horizontal orbital microplate shaker.
06
Wash the plate and add 100μl of HRP conjugate to each well.
07
Incubate for 1 hour at room temperature on a horizontal orbital microplate shaker.
08
Wash the plate and add 100μl of TMB substrate to each well.
09
Incubate for 0.5 hours at room temperature in the dark.
10
Stop the reaction by adding 50μl of Stop Solution to each well.
11
Read the absorbance at the appropriate wavelength within 10 minutes.
Who needs raybio humanmouserat urotensin- ii?
01
Researchers studying the physiology and pathophysiology of urotensin-II in humans, mice, or rats.
02
Scientists investigating the role of urotensin-II in cardiovascular diseases.
03
Laboratories developing diagnostic assays for urotensin-II detection.
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What is raybio humanmouserat urotensin- ii?
RayBio Human/Mouse/Rat Urotensin-II is a type of protein that is often studied in the context of cardiovascular and metabolic research due to its role in regulating blood pressure and other physiological functions.
Who is required to file raybio humanmouserat urotensin- ii?
Typically, researchers or laboratories conducting studies on Urotensin-II in human, mouse, or rat specimens are required to file the relevant studies or notifications.
How to fill out raybio humanmouserat urotensin- ii?
Filling out the RayBio human/mouse/rat urotensin-II documentation usually involves providing details of the study protocol, sample collection methods, and data analysis procedures. Specific guidelines should be followed based on the consulting regulations.
What is the purpose of raybio humanmouserat urotensin- ii?
The purpose of RayBio Human/Mouse/Rat Urotensin-II is mainly for research into its biological effects and potential therapeutic applications related to cardiovascular health and metabolic disorders.
What information must be reported on raybio humanmouserat urotensin- ii?
Reported information usually includes study identifiers, methodology, results, interpretation of data, and any observed effects related to Urotensin-II across different species.
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