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EPIDEMIOLOGY AND SURVEILLANCEcrossm Escherichia coli Harboring mcr1 in a Cluster of Liver Transplant Recipients: Detection through Active Surveillance and Wholesome SequencingaDivision of Infectious
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Begin by preparing the LB agar plates.
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Take a colony of Escherichia coli harboring mcr-1 and streak it onto the LB agar plate.
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Incubate the plate at the appropriate temperature (usually 37 degrees Celsius) for several hours.
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Select a single colony from the plate and inoculate it into LB broth.
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Allow the culture to grow overnight with shaking.
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Perform any required analysis or experimentation with the Escherichia coli harboring mcr-1.

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Researchers studying antibiotic resistance in Escherichia coli may need Escherichia coli harboring mcr-1 for their experiments.
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Medical professionals researching treatments for mcr-1 carrying bacteria could also benefit from using Escherichia coli harboring mcr-1.
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Escherichia coli harboring mcr-1 is a strain of E. coli that possesses the mcr-1 gene, which provides resistance to colistin, an antibiotic that is often used as a last resort to treat multi-drug resistant infections.
Healthcare facilities, laboratories, and public health organizations that identify cases of E. coli harboring the mcr-1 gene are required to file reports to monitoring agencies as part of surveillance for antibiotic resistance.
To fill out reporting forms for E. coli harboring mcr-1, one should include patient identification, laboratory results confirming the presence of mcr-1, and any relevant treatment information, following the guidelines provided by the reporting authority.
The purpose of monitoring E. coli harboring mcr-1 is to track the spread of antibiotic resistance, which is critical for public health efforts to control infections and inform treatment guidelines.
The report should include the isolate's identification details, testing results showing mcr-1 presence, demographics of the patient, clinical outcomes, and any antibiotic treatment administered.
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