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Version 090909 G:productsproductflyersiderophoresdata_sidero_sum.docx data_sidero_sum.DOCX EXXON b i o s c i e n c e ___ for: +49 (0)731 360 812 3fax: +49 (0)731 360 8 962eMail: info@genaxxon.com internet: www.genaxxon.comSiderophores Videophones ores

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How to fill out bloodgenomic dna purification mini

01

Thaw the Proteinase K and Buffer BL at room temperature.

02

Add 180 ul of Buffer BL to the thawed Proteinase K.

03

Purge the E为agarose columns, collection tubes and buffers provided in the kit.

04

Add 400 ul of whole blood to a 1.5 ml microcentrifuge tube.

05

Add 20 ul of Proteinase K/Buffer BL mixture to the whole blood sample.

06

Incubate at 56°C for 10 minutes, mixing by pulse-vortexing every 2 minutes.

07

Add 200 ul of 100% ethanol to the sample and mix thoroughly by pulse-vortexing.

08

Transfer the mixture to the E为agarose spin column placed in a collection tube, and centrifuge for 1 minute at 8,000 x g.

09

Discard the flow through and place the E为agarose column back in the collection tube.

10

Wash the E为agarose column with Wash Buffer I by centrifuging for 1 minute at 8,000 x g.

11

Wash the E为agarose column with Wash Buffer II by centrifuging for 3 minutes at 8,000 x g.

12

Place the E为agarose column in a clean microcentrifuge tube, and add Elution Buffer to the center of the column membrane.

13

Incubate for 3 minutes, then centrifuge for 2 minutes at 8,000 x g to elute the DNA.

Who needs bloodgenomic dna purification mini?

01

Researchers working in molecular biology laboratories who need to purify genomic DNA from blood samples for various downstream applications such as PCR, sequencing, and genotyping.

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What is bloodgenomic dna purification mini?

Bloodgenomic DNA purification mini refers to a specialized procedure and kit designed for the efficient extraction and purification of DNA from blood samples for analytical and research purposes.

Who is required to file bloodgenomic dna purification mini?

Individuals or laboratories involved in genetic research, clinical diagnostics, or any projects necessitating DNA extraction from blood samples are typically required to file bloodgenomic DNA purification mini.

How to fill out bloodgenomic dna purification mini?

Filling out bloodgenomic DNA purification mini involves providing necessary information such as sample identification, extraction protocols followed, and any relevant laboratory results obtained.

What is the purpose of bloodgenomic dna purification mini?

The purpose of bloodgenomic DNA purification mini is to isolate DNA from blood samples efficiently, enabling further analysis, research, or clinical testing related to genetics and genomics.

What information must be reported on bloodgenomic dna purification mini?

Information that must be reported includes sample ID, extraction method, quality and quantity of DNA obtained, and any observational data relevant to the sample handling.

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