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Human WNT3A ELISA Kit Catalog NO.: RK04267version: 2.0 This package insert must be read in its entirety before using this product1www.ab clonal.introduction The kit is a sandwich enzyme immunoassay
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Step 1: Prepare all necessary materials including samples, standards, diluent buffer, wash buffer, detection antibody, substrate solution, stop solution, and microwell plate coated with TNF-alpha antibody.
02
Step 2: Dilute the detection antibody in diluent buffer according to the kit instructions.
03
Step 3: Add samples, standards, and diluted detection antibody to appropriate wells in the microwell plate.
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Step 4: Incubate the plate for a specified amount of time at the recommended temperature.
05
Step 5: Wash the plate to remove any unbound substances.
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Step 6: Add substrate solution to each well and incubate again for a specific amount of time.
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Step 7: Stop the reaction by adding stop solution.
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Step 8: Measure the absorbance of each well using a microplate reader.
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Step 9: Calculate the concentration of TNF-alpha in the samples using the standard curve provided in the kit.

Who needs human tnf-alpha elisa kit-human?

01
Researchers studying inflammatory diseases like rheumatoid arthritis or Crohn's disease may need the human TNF-alpha ELISA kit for measuring TNF-alpha levels in biological samples.
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Clinicians monitoring patients with autoimmune disorders or those undergoing treatment with TNF-alpha inhibitors may also require the kit to assess TNF-alpha levels.
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The human TNF-alpha ELISA kit is a laboratory tool used for measuring the levels of tumor necrosis factor alpha (TNF-alpha) in human samples, typically serum or plasma.
Researchers, clinicians, or laboratory technicians involved in studies related to inflammation, autoimmune diseases, or other conditions affected by TNF-alpha levels are required to utilize this ELISA kit.
Filling out the human TNF-alpha ELISA kit involves following the manufacturer's specific instructions for sample preparation, incubation times, and measurement procedures, which typically include adding samples to wells, mixing with reagents, and reading the absorbance.
The purpose of the human TNF-alpha ELISA kit is to quantify TNF-alpha levels for research and diagnostic purposes, aiding in the understanding and management of inflammatory diseases.
Information such as sample identification, assay results, concentrations of TNF-alpha, and any relevant patient or sample data must be reported when using the kit.
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