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RESEARCH ARTICLEUsing de novo assembly to identify structural variation of eight complex immune system gene regions JiaYuan Zhang ID1*, Hannah Roberts ID1, David S. C. Flores1, Antony J. Cutler ID1,
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How to fill out using de novo assembly
How to fill out using de novo assembly
01
Extract DNA or RNA sequences from the organism of interest.
02
Sequence the DNA or RNA using next generation sequencing technology.
03
Assemble the short sequencing reads into longer contiguous sequences using bioinformatics software.
04
Annotation of the assembled sequences to identify genes and functional elements.
Who needs using de novo assembly?
01
Biologists studying non-model organisms without a reference genome.
02
Researchers trying to identify novel genes or genetic variations.
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What is using de novo assembly?
De novo assembly is a method used in bioinformatics to assemble DNA sequences from short reads without a reference genome.
Who is required to file using de novo assembly?
Researchers and organizations conducting genomic research that necessitates custom genome assembly are typically required to file using de novo assembly.
How to fill out using de novo assembly?
To fill out using de novo assembly, one should provide the assembled sequences, metadata, and any relevant annotations following the specific submission guidelines.
What is the purpose of using de novo assembly?
The purpose of using de novo assembly is to create complete genomes from short, fragmented DNA sequences when no reference genome is available.
What information must be reported on using de novo assembly?
Information that must be reported includes the assembled sequences, sample origin, sequencing technology used, and any quality control measures taken.
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