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J. Crop Prot. 2020, 9 (4): 683697___Downloaded from jcp.modares.ac.ir at 10:21 IRST on Monday January 11th 2021Research ArticleIncidence and distribution of Tobacco streak virus isolated from parsley
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How to fill out tobacco necrosis virus-a c

01
Obtain a sample of tobacco tissue infected with tobacco necrosis virus-a c.
02
Extract the RNA from the tobacco tissue sample using a suitable extraction kit.
03
Prepare a reverse transcription reaction to convert the extracted RNA into cDNA.
04
Amplify the cDNA using polymerase chain reaction (PCR) with specific primers for tobacco necrosis virus-a c.
05
Analyze the PCR products on an agarose gel to confirm the presence of the virus.
06
Optional: Sequence the PCR products to further characterize the virus.

Who needs tobacco necrosis virus-a c?

01
Researchers studying plant virology.
02
Agricultural professionals looking to diagnose and monitor tobacco necrosis virus-a c infections in tobacco crops.
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Tobacco necrosis virus-a c is a virus that primarily affects plants, particularly tobacco, causing tissue necrosis and various symptoms that can lead to significant agricultural losses.
Growers and suppliers of tobacco products who are involved in the cultivation or distribution of tobacco plants affected by tobacco necrosis virus-a c are required to file relevant reports.
To fill out tobacco necrosis virus-a c, individuals must provide accurate information regarding the affected plants, their location, and any measures taken to control the virus, following the specific guidelines laid out by agricultural authorities.
The purpose of tobacco necrosis virus-a c is to track the occurrence of the virus, implement control measures, and prevent the spread of the virus in agricultural settings.
Information that must be reported includes details of the infected plants, the symptoms observed, geographical location, and steps taken for management and control of the virus.
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