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General protocol for ENZYME IMMUNOASSAY KIT (range: 0100ng/ml)PHOENIX PHARMACEUTICALS, INC.INTRODUCTION This Enzyme Immunoassay kit is designed to detect a specific peptide and its related peptides
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01
Prepare the samples by diluting them as necessary.
02
Add the samples to the wells of the assay plate.
03
Incubate the plate to allow the target molecules to bind to the capture antibodies on the plate.
04
Wash the plate to remove unbound substances.
05
Add the detection antibodies and any necessary substrates to the wells.
06
Incubate the plate again to allow the detection antibodies to bind to the target molecules.
07
Wash the plate to remove any unbound detection antibodies.
08
Measure the signal produced by the bound detection antibodies to quantify the target molecules.

Who needs immunoassay methods - assay?

01
Researchers in the fields of immunology, biochemistry, and pharmacology who are studying the presence and levels of specific proteins or antibodies in biological samples.
02
Clinical laboratories that need to diagnose diseases, monitor the progression of diseases, or determine the effectiveness of treatments.
03
Pharmaceutical companies developing new drugs that require the measurement of specific biomarkers in preclinical and clinical studies.
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Immunoassay methods are laboratory techniques used to measure the presence or concentration of a substance, usually in a biological sample, through the use of an antigen-antibody reaction.
Typically, laboratories and healthcare providers utilizing immunoassay methods for diagnostic purposes are required to file relevant documentation and results.
To fill out immunoassay methods documentation, practitioners must provide specific details about the assay protocol, results, controls used, and interpretations based on the findings.
The purpose of immunoassay methods is to detect and quantify substances like hormones, drugs, or proteins in various sample types, aiding in diagnosis and monitoring of diseases.
Information that must be reported includes the assay type, results, sample identification, controls, calibration details, and any relevant clinical interpretations.
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