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US010733614B2States Patent (12) United Saponin et al.(10) Patent No.: US 10,733,614 B2 *Aug. 4, 2020 (45) Date of Patent: (58) Field of Classification Search CPC G06Q 30/016 ; G06Q 10/1053 ; G06Q 10/107

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How to fill out a hybrid open-top light-sheet

01

Prepare the sample and mount it on a glass coverslip.

02

Secure the coverslip on the sample holder of the hybrid open-top light-sheet setup.

03

Adjust the lasers and lenses to achieve desired illumination and imaging conditions.

04

Start the imaging process by turning on the light-sheet and camera.

05

Capture images of the sample using the hybrid open-top light-sheet.

Who needs a hybrid open-top light-sheet?

01

Researchers in the field of developmental biology who need to visualize the dynamic processes within live organisms at high resolution.

02

Scientists studying cell biology who require gentle imaging techniques to minimize photodamage to the sample.

03

Biologists investigating three-dimensional structures of tissues and organs in transparent organisms or cleared samples.

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What is a hybrid open-top light-sheet?

A hybrid open-top light-sheet is a specific type of information return used by certain entities to report their financial data and operational activities, combining elements of both traditional and innovative reporting requirements.

Who is required to file a hybrid open-top light-sheet?

Entities that meet specific criteria, such as certain businesses or organizations engaging in defined activities, are required to file a hybrid open-top light-sheet.

How to fill out a hybrid open-top light-sheet?

To fill out a hybrid open-top light-sheet, eligible entities must gather their financial information, input necessary data into the appropriate sections, and ensure compliance with all filing instructions provided.

What is the purpose of a hybrid open-top light-sheet?

The purpose of a hybrid open-top light-sheet is to provide regulatory authorities with detailed and structured information regarding an entity's financial health and operational processes, thus ensuring transparency and accountability.

What information must be reported on a hybrid open-top light-sheet?

The hybrid open-top light-sheet requires reporting information such as revenues, expenses, asset valuations, operational activities, and any other relevant financial metrics as dictated by regulatory standards.

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