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Experiment 17Emission and Absorption Spectroscopy PreLab Assignment Before coming to lab: Read the lab thoroughly. Answer the prelab questions that appear at the end of this lab exercise.Purpose A

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How to fill out 52 beers law and

01

Measure the absorbance of a series of standard solutions with known concentrations of a substance.

02

Plot a graph of absorbance versus concentration.

03

Find the slope of the line on the graph, which is equal to the molar absorptivity (molar extinction coefficient) of the substance.

04

Use the Beer-Lambert law equation, A = εbc, where A is the absorbance, ε is the molar absorptivity, b is the path length of the cuvette, and c is the concentration, to calculate the concentration of an unknown sample.

Who needs 52 beers law and?

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Chemists and researchers who need to determine the concentration of a substance in a solution can use the Beer's Law equation.

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Pharmaceutical companies may also use Beer's Law in the development and testing of drugs to quantify the concentration of active ingredients in formulations.

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The 52 Beers Law refers to regulatory requirements in the United States for certain types of businesses involved in the sale or distribution of alcoholic beverages, mandating them to report specific information to federal authorities.

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