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NGS Course Nove Hrady Documentation Release 1.0Libor Morkovsky, Vaclav JanousekJan 22, 2019Contents1Installation instructions32Connecting to the virtual machine93Unix Basics134Unix Advanced I215Unix
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How to fill out next generation sequencing approaches

01
Extract DNA or RNA from the sample of interest
02
Prepare the library by fragmenting the DNA/RNA and adding adapters
03
Amplify the library using PCR to increase the amount of material
04
Sequencing the library using a next generation sequencing platform
05
Analyze the resulting data to identify genetic variations or gene expression levels

Who needs next generation sequencing approaches?

01
Researchers studying genetic diseases or mutations
02
Clinicians diagnosing hereditary conditions
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Pharmaceutical companies developing personalized medicine
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Agricultural biotechnologists improving crop yields
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Forensic scientists identifying suspects using DNA evidence
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Next generation sequencing (NGS) approaches refer to modern sequencing technologies that allow for the rapid sequencing of large stretches of DNA base pairs. These methods enable the analysis of whole genomes, targeted regions, or transcriptomes, providing high-throughput and accurate Genomic data.
Typically, researchers, genetic testing laboratories, and companies involved in genomics and biotechnology are required to file next generation sequencing approaches, especially if they are reporting results for regulatory compliance or clinical assessments.
Filling out next generation sequencing approaches generally involves providing detailed information on the sequencing methods used, the samples analyzed, the data generated, and any interpretations or findings derived from the sequencing process.
The purpose of next generation sequencing approaches is to enable comprehensive genomic analysis in a time-efficient and cost-effective manner, allowing for applications in medical diagnostics, personalized medicine, cancer research, and evolutionary studies.
Information that must be reported typically includes sequencing methodology, sample identification, assay performance metrics, variant calling, and any interpretations relevant to clinical or research outcomes.
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