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2019 SCIENTIFIC MEETING Signalling & Gene Regulation in Health & Disease BABRAHAM INSTITUTE | CAMBRIDGE, 1819th NOV 20192019 EULife Scientific Meeting | Babraham Institute | Cambridge, 1819th NOV
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How to fill out long-range enhancer-promoter contacts in

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How to fill out long-range enhancer-promoter contacts in

01
Identify the specific long-range enhancer and promoter regions in the genome.
02
Determine the experimental methods to detect the interactions between enhancers and promoters.
03
Perform chromatin conformation capture (3C) or Hi-C experiments to capture physical interactions.
04
Analyze the data to identify significant enhancer-promoter contacts.
05
Validate the identified contacts using techniques like ChIP-qPCR or reporter assays.

Who needs long-range enhancer-promoter contacts in?

01
Researchers studying gene regulation mechanisms and transcriptional control
02
Scientists interested in understanding the spatial organization of the genome
03
Drug developers looking to target specific gene regulatory elements for therapeutic purposes
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Long-range enhancer-promoter contacts refer to the interactions between enhancer regions and promoter regions of a gene that can be located far apart on the same chromosome. These contacts are critical for the regulation of gene expression.
Typically, researchers and geneticists who are involved in studies related to gene regulation and expression in various organisms are required to file long-range enhancer-promoter contacts in.
To fill out long-range enhancer-promoter contacts, one must collect data on enhancer and promoter locations, document interactions using techniques like 3C or Hi-C, and then submit this information in the specified format to the appropriate database or regulatory body.
The purpose of long-range enhancer-promoter contacts is to understand gene regulation mechanisms, how different genes are turned on or off, and to explore the implications for health, disease, and developmental biology.
Information that must be reported includes the specific enhancer and promoter sequences, their genomic locations, the nature of their interactions, and any experimental methods used to determine these contacts.
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