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Open access, freely available onlinePLoS BIOLOGYPatterns of Intron Gain and Loss in Fungi Cydney B. Nielsen1,2[, Brad Friedman1,3[, Bruce Birren2, Christopher B. Burge1*, James E. Galagan2* 1 Department
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How to fill out patterns of intron gain

01
Identify the sequences flanking the insertion site of the intron
02
Align these flanking sequences with homologous sequences from related species to determine if the intron is conserved
03
Check for the presence of consensus sequences at the splice sites
04
Verify if the intron interrupts the reading frame of the gene
05
Document the location and content of the intron for further analysis

Who needs patterns of intron gain?

01
Researchers studying the evolution of gene structure
02
Scientists interested in understanding the molecular mechanisms behind intron gain
03
Genomic analysts looking to identify patterns of intron gain across species
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Patterns of intron gain refer to the evolutionary events where introns are gained within a coding sequence of a gene.
Researchers and scientists studying genetic evolution are typically required to file patterns of intron gain.
Patterns of intron gain can be filled out by documenting the coding sequence of a gene and highlighting any newly gained introns.
The purpose of documenting patterns of intron gain is to better understand the evolution of genes and genetic sequences.
Information such as the gene name, genomic location, sequence changes, and evolutionary context must be reported on patterns of intron gain.
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