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8Forests
Balance:In the last unit, students learned how complex
living things are made up of specialized cells,
tissues, organs, and organ systems that have
particular functions. In this unit, they
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01
Start by gathering all the necessary materials for cell culture technique, including cell culture dishes, growth medium, trypsin-EDTA solution, pipettes, and a cell counter.
02
Thaw the required cells from frozen stock by quickly transferring to a 37°C water bath until nearly thawed, then transfer to a culture hood for further preparation.
03
Prepare the cell culture dishes by coating with extracellular matrix proteins if needed, and then add the appropriate amount of growth medium to the dish.
04
Add the cells to the culture dish and place in a cell culture incubator set to the appropriate temperature and CO2 concentration for cell type being cultured.
05
Monitor the cells regularly under a microscope to ensure proper growth and health, changing the medium as needed and subculturing when the cells reach confluency.
Who needs 1 explain how cells?
01
Researchers working in fields such as molecular biology, pharmaceuticals, regenerative medicine, and biotechnology need to understand how cells work and interact in order to advance their research and develop new treatments.
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1 cells are the basic unit of structure and function in living organisms. They contain genetic material and perform specific functions within the body.
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