Get the free WiCell recommends thawing 1 vial into 2 wells of a 6 well plate using TeSR-E8 and Re...
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Thaw and Culture DetailsCell Line Name
Well Lot Number
Parent Material
Provider
Banked By
Thaw and Culture
Recommendations
ProtocolJHU214i
WB67611
JHU214i DB36851
Johns Hopkins University Laboratory
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01
Thaw Wicell recommends thawing 1 vial by removing it from the liquid nitrogen storage tank and quickly transferring it to a 37°C water bath.
02
Shake the vial gently until all the ice crystals are melted.
03
Do not leave the vial in the water bath for more than 2 minutes.
04
Quickly dry the vial with a paper towel to prevent water from getting inside.
05
Once thawed, use the cells immediately or store them in liquid nitrogen for future use.
Who needs wicell recommends thawing 1?
01
Researchers working with Wicell cell lines that require thawing before use.
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What is wicell recommends thawing 1?
WiCell recommends thawing 1 is a specific protocol or set of instructions for thawing a particular type of cells stored in a cryogenic freezer.
Who is required to file wicell recommends thawing 1?
Researchers or laboratory technicians working with the specific type of cells mentioned in wicell recommends thawing 1 protocol are required to follow and file it accordingly.
How to fill out wicell recommends thawing 1?
To fill out wicell recommends thawing 1, one needs to carefully follow the step-by-step instructions provided in the protocol, ensuring that each step is accurately executed.
What is the purpose of wicell recommends thawing 1?
The purpose of wicell recommends thawing 1 is to ensure the successful and proper thawing of cells from cryogenic storage, maintaining their viability and functionality for further research or applications.
What information must be reported on wicell recommends thawing 1?
The information reported on wicell recommends thawing 1 includes details of the specific cells being thawed, the date and time of thawing, the individual performing the thawing process, any observations or notes during the thawing process, and any post-thawing instructions or recommendations.
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