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NETFLIX Rapid Directional RNAS Automation Kit 2.0 (For Illumine & Element Platforms) NOVA519853NEXTFLEX Rapid Directional RNAS Automation Kit 2.0 GENERAL INFORMATION ___ 3 Product Overview ___ 3 Provided
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01
Thaw the RNA on ice before use.
02
Combine the RNA with the provided reaction mix and nuclease-free water.
03
Incubate the reaction mixture at 70°C for 5 minutes, then cool down to 42°C.
04
Add the reverse transcriptase enzyme and mix thoroughly.
05
Incubate the reaction at 42°C for 15 minutes for cDNA synthesis.
06
Add the first strand synthesis buffer and second strand synthesis enzyme to the reaction mix.
07
Incubate the reaction at 16°C for 1 hour for second strand synthesis.
08
Add the provided end repair enzyme mix and incubate at 20°C for 30 minutes.
09
Add the ligation mix and incubate at 20°C for 15 minutes for ligation.
10
Proceed with library purification and quantification steps before sequencing.
Who needs nextflex rapid directional rna-seq?
01
Researchers conducting gene expression studies.
02
Scientists studying RNA splicing or gene regulation.
03
Laboratories interested in differential gene expression analysis.
04
Individuals working on identifying novel RNA transcripts or isoforms.
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What is nextflex rapid directional rna-seq?
NextFlex Rapid Directional RNA-Seq is a high-throughput sequencing method for analyzing gene expression levels.
Who is required to file nextflex rapid directional rna-seq?
Researchers or laboratories conducting gene expression studies may be required to file NextFlex Rapid Directional RNA-Seq.
How to fill out nextflex rapid directional rna-seq?
NextFlex Rapid Directional RNA-Seq can be filled out by following the specific protocol provided by the kit manufacturer.
What is the purpose of nextflex rapid directional rna-seq?
The purpose of NextFlex Rapid Directional RNA-Seq is to measure gene expression levels in a high-throughput manner.
What information must be reported on nextflex rapid directional rna-seq?
NextFlex Rapid Directional RNA-Seq typically requires reporting of gene expression levels for different samples.
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