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MMS TRANSGENIC ANIMAL MODELING CORE Service Statement In Vitro Fertilization CORE WILL PROVIDE: 1. Collection and culture of sperm from transgenic male mice, supplied by investigator. 2. Insemination
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01
Prepare all necessary materials like tamc media, mouse eggs, sperm, and culture dishes.
02
Thaw the tamc media and warm it to the appropriate temperature.
03
Collect the mouse eggs and sperm from donor mice and prepare them for fertilization.
04
Place the mouse eggs and sperm together in a culture dish containing the tamc media.
05
Incubate the culture dish at the appropriate temperature and for the appropriate amount of time.
06
Check for successful fertilization and embryo development under a microscope.
07
Transfer the developed embryos to a recipient female mouse for implantation and gestation.

Who needs tamc - mouse ivf?

01
Researchers and scientists working in the field of mouse reproductive biology and assisted reproductive technologies.
02
Those interested in studying embryonic development, genetics, and gene editing in mice.
03
Individuals looking to produce genetically modified mice for research purposes.
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tamc - mouse ivf stands for Transgenic Animal Model Core - Mouse In Vitro Fertilization.
Researchers or institutions conducting mouse in vitro fertilization experiments are required to file tamc - mouse ivf.
tamc - mouse ivf must be filled out with detailed information about the research project, including the purpose, methods, and expected outcomes.
The purpose of tamc - mouse ivf is to track and regulate experiments involving mouse in vitro fertilization for research purposes.
Information such as project title, investigator details, experimental procedures, and expected results must be reported on tamc - mouse ivf.
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Relevant keywords

umass umms c57bl sjl f1 biopsies transgenics cryopreserve motile transgenic weaning designation fertilization gestation pseudo fertilized progeny forego insemination vitro lineage insufficient strains elect inquire quantity

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