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ANTICANCER RESEARCH 35: 64876496 (2015)Coexpression of Cytoskeleton Protein Adducing 3 and
CD133 in Exospheres and a Temozolomideresistant
Subclone of Nuroblastoma
QINGHAI POON×, JAMES TIN LONG ZHUANG×,
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How to fill out co-expression of cytoskeletal protein
01
Identify the cytoskeletal proteins of interest that you want to co-express.
02
Select a suitable expression system such as bacterial, yeast, or mammalian cells.
03
Design and clone the expression constructs for each cytoskeletal protein, ensuring appropriate tags or markers for detection.
04
Transfect or transform the expression constructs into the chosen host cells.
05
Cultivate the cells under suitable conditions to allow for protein expression and co-localization studies.
06
Perform co-immunoprecipitation or immunofluorescence assays to confirm the co-expression and interaction of the cytoskeletal proteins.
Who needs co-expression of cytoskeletal protein?
01
Researchers studying cytoskeletal dynamics and functions.
02
Scientists investigating the role of cytoskeletal proteins in various cellular processes such as cell migration, division, and signaling.
03
Drug developers looking to target cytoskeletal elements for therapeutic purposes.
04
Biomedical researchers interested in understanding diseases associated with cytoskeletal abnormalities.
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What is co-expression of cytoskeletal protein?
Co-expression of cytoskeletal proteins refers to the simultaneous expression of two or more proteins that make up the cytoskeleton in a cell, allowing researchers to study their interactions and collective functions.
Who is required to file co-expression of cytoskeletal protein?
Researchers and organizations conducting experiments involving cytoskeletal proteins and their co-expression may be required to file relevant documentation depending on institutional and governmental regulations.
How to fill out co-expression of cytoskeletal protein?
To fill out the co-expression documentation, one typically needs to provide details about the proteins involved, their expression levels, experimental conditions, and any relevant methodologies used in the study.
What is the purpose of co-expression of cytoskeletal protein?
The purpose of studying co-expression of cytoskeletal proteins is to understand their roles in cellular functions, interactions, and how they contribute to various biological processes and diseases.
What information must be reported on co-expression of cytoskeletal protein?
Information that must be reported includes the specific proteins being co-expressed, their functional relevance, expression levels, experimental conditions, and data interpretation.
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