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Procedure & Checklist (Modified from: \”Preparing Smell Libraries using Pablo Barcoded Universal Primers for Multiplexing Amplicons\”) Before You Being Digested and purified barcoded RNA fragments
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Preparing smrtbell libraries involves the process of creating molecular libraries that allow for the efficient sequencing of DNA using the smrt sequencing technology.
Researchers and institutions that are utilizing smrt sequencing technology for their DNA analysis are required to prepare and file smrtbell libraries.
To fill out the preparation for smrtbell libraries, one must follow the specific protocols outlined by the sequencing technology provided by Pacific Biosciences, which include guidelines on sample input, fragmentation, and ligation.
The purpose of preparing smrtbell libraries is to generate high-quality DNA libraries that can be used for long-read sequencing, improving the accuracy and efficiency of genomic studies.
Information that must be reported includes the type and amount of input DNA, the library preparation protocol used, and any relevant quality control metrics.
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