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MOLECULAR BIOLOGY/GENOMICSCytochrome c Gene and Protein Expression: Developmental Regulation, Environmental Response, and Pesticide Sensitivity in Aedes aegypti LIMING ZHAO,1 JULIA W. PRIDGEON, JAMES J. BECNEL, GARY G. CLARK, AND KENNETH J. LINTHICUM Center for Medical, Agricultural, and Veterinary Entomology, USDAARS, 1600 SW 23rd Drive, Gainesville, FL 32608 USAJ. Med. Entomol. 45(3): 401408 (2008)ABSTRACT Cytochrome c is a highly conserved protein that is found in many multicellular and...
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How to fill out cytochrome c gene and
How to fill out cytochrome c gene and
01
Start with obtaining the cDNA or genomic DNA sample containing the cytochrome c gene.
02
Prepare the PCR reaction mixture including DNA polymerase, primers specific to cytochrome c, dNTPs, and buffer.
03
Set the PCR conditions: Initial denaturation at 95°C for 2 minutes, followed by 30 cycles of denaturation at 95°C for 30 seconds, annealing at the specific primer temperature for 30 seconds, and extension at 72°C for 1 minute.
04
Visualize the PCR products using gel electrophoresis to ensure the amplification of the cytochrome c gene.
05
If necessary, clone the PCR product into a vector for further studies or sequencing.
Who needs cytochrome c gene and?
01
Researchers studying mitochondrial function and energy metabolism.
02
Biomedical scientists investigating the role of cytochrome c in apoptosis.
03
Geneticists analyzing mitochondrial DNA disorders.
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Pharmaceutical companies developing drugs targeting mitochondrial pathways.
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What is cytochrome c gene?
The cytochrome c gene encodes a protein that plays a critical role in the electron transport chain, which is part of cellular respiration.
Who is required to file cytochrome c gene?
Researchers in genetics and molecular biology, as well as entities involved in studying mitochondrial functions, may be required to file information regarding the cytochrome c gene.
How to fill out cytochrome c gene?
To fill out information about the cytochrome c gene, one typically needs to gather data on gene sequences, expression levels, and functional assays.
What is the purpose of cytochrome c gene?
The purpose of the cytochrome c gene is to code for cytochrome c protein, which is essential for electron transport, energy production, and apoptosis regulation in cells.
What information must be reported on cytochrome c gene?
Information that must be reported includes gene sequence, mutations, expression patterns, functional roles, and associations with diseases.
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