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DOCUMENTS DE COTIZACIN SPAS No. 2021ROOC299ADQUISICIN DE MULTIPLEX PCR EN TEMPO REAL, PARA EL HOSPITAL ROOSEVELT: 16078020Guatemala, December Del AO 20211. TERMINOLOGA1.1 NEXT (S) Apart ado de Estes
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La optimización de la PCR multiplex es un proceso que permite amplificar múltiples secuencias de ADN simultáneamente en una sola reacción, mejorando la eficiencia y reduciendo el tiempo de análisis.
Cualquier investigador o laboratorio que desee realizar análisis genéticos que involucren múltiples objetivos de ADN debe presentar la optimización de PCR multiplex.
Se completa siguiendo una serie de pasos que incluyen la selección de cebadores adecuados, la calibración de las condiciones de la PCR, y la validación de los resultados a través de controles adecuados.
El propósito es maximizar la eficacia de la amplificación de múltiples secuencias genéticas simultáneamente, garantizando resultados confiables y reproducibles.
Se debe reportar la secuencia de los cebadores, las condiciones de la reacción, los resultados de las pruebas de amplificación y la especificidad de los productos generados.
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