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Page 1 of 171.(a)Describe and explain how the polymerase chain reaction (PCR) is used to amplify a DNA fragment. ___ ___ ___ ___ ___ ___ ___ ___ ___ (4)The figure below shows the number of DNA molecules
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01
Prepare your sample by extracting DNA/RNA.
02
Set up a PCR reaction mixture, including DNA template, primers, nucleotides, DNA polymerase, and buffer.
03
Place the reaction mixture in a PCR tube.
04
Program the PCR machine with the following cycles: initial denaturation, denaturation, annealing, extension, and final extension.
05
Run the PCR machine according to the programmed settings.
06
Analyze the PCR products using gel electrophoresis.

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Clinicians for diagnostic testing.
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Forensic scientists for crime scene analysis.
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Agricultural scientists for GMO testing.
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Laboratory technicians for genetic studies.
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Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA sequences, allowing scientists to generate millions of copies of a particular DNA segment from a small sample.
Typically, individuals or entities involved in genetic research, clinical laboratories, or organizations conducting DNA analyses may be required to file PCR results, depending on regulatory requirements.
Filling out PCR documentation usually involves providing details such as sample information, protocols used, results obtained, and any annotations related to the analysis.
The primary purpose of PCR is to amplify DNA for various applications, including genetic testing, cloning, forensic analysis, and studying genetic variations.
Information that must be reported typically includes the DNA sample identification, method used, amplification results, and any pertinent observations regarding the experiment.
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