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HumanIL21 Immunoassay Catalog Number: SEKH0032For the quantitative determination of human interleukin21 (IL21) concentrations in cellculture supernates, serum, and plasma.For research use only. Not
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01
Collect a blood sample from the patient following standard venipuncture procedures.
02
Transfer the blood sample to a sterile tube and label it appropriately.
03
Centrifuge the sample to separate the serum or plasma from the blood cells, following the manufacturer's guidelines.
04
Store the serum or plasma at -80°C until ready for analysis.
05
Utilize an ELISA kit specifically designed for measuring IL-21 levels, following the protocol provided by the manufacturer.
06
Prepare the reagents as instructed and add the samples to the assay plate.
07
Incubate the plate for the specified time and temperature to allow for binding.
08
Wash the plate to remove unbound substances and add the detection reagents.
09
Measure the absorbance using a microplate reader at the designated wavelength.
10
Analyze the results and compare them to standard curves to quantify IL-21 levels.

Who needs measurement of il-21 in?

01
Patients with autoimmune disorders to assess immune dysregulation.
02
Researchers studying inflammatory diseases and cytokine profiles.
03
Patients undergoing treatments that may affect immune function, to monitor responses.
04
Individuals with chronic infections where IL-21 levels may influence disease outcomes.
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Measurement of IL-21 refers to the assessment or quantification of the interleukin-21 cytokine in biological samples, which is important for understanding immune responses.
Entities conducting research or clinical trials involving IL-21 should file measurements, including researchers, institutions, and laboratories.
To fill out the measurement of IL-21, one typically needs to collect samples, perform assays to determine IL-21 levels, and document the results in a standardized format.
The purpose of measuring IL-21 is to evaluate immune responses, research autoimmune diseases, and assess the efficacy of treatments targeting immune modulation.
Information reported typically includes sample identification, assay methodology, IL-21 concentration levels, and any relevant subject or study identifiers.
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