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University of WindsorScholarship at UWindsor Electronic Theses and DissertationsTheses, Dissertations, and Major Papers2013Surface Enhanced Raman Scattering and Fluorescence of Tagged Phospholipids
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01
Prepare the sample: Ensure that the analyte (molecule of interest) is in solution or on a suitable substrate.
02
Choose a SERS-active substrate: Use materials like metallic nanoparticles (gold or silver) for optimal enhancement.
03
Apply the sample: Add a small amount of the analyte solution to the SERS substrate, allowing it to adsorb onto the surface.
04
Dry the sample: If necessary, allow the sample to air dry or use a gentle heat source to remove excess solvent without damaging the analyte.
05
Set up the Raman spectrometer: Calibrate the instrument according to the manufacturer’s guidelines and ensure the laser wavelength is appropriate for your sample.
06
Collect data: Focus the laser on the substrate and record the surface-enhanced Raman scattering spectra.
07
Analyze the spectra: Compare the collected data with reference spectra to interpret the results and identify the analyte.
Who needs surface enhanced raman scattering?
01
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What is surface enhanced raman scattering?
Surface Enhanced Raman Scattering (SERS) is a technique that enhances the Raman scattering of molecules adsorbed on rough metal surfaces or nanoparticles, allowing for the detection of low concentrations of substances.
Who is required to file surface enhanced raman scattering?
Filing for Surface Enhanced Raman Scattering is typically not required; however, researchers and companies involved in nanotechnology and spectroscopy may need to comply with specific regulations depending on their work.
How to fill out surface enhanced raman scattering?
The process of filling out documentation related to Surface Enhanced Raman Scattering depends on the specific regulatory framework and can vary by jurisdiction; typically involves detailing the methodologies, results, and applications of the technique.
What is the purpose of surface enhanced raman scattering?
The purpose of Surface Enhanced Raman Scattering is to increase the sensitivity of Raman spectroscopy, allowing for the identification and quantification of molecules at very low concentrations.
What information must be reported on surface enhanced raman scattering?
Information typically reported includes the experimental setup, materials used, the enhancement factors observed, and the specific applications of the findings.
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