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SEA052Hu 96 Tests Enzymelinked Immunosorbent Assay Kit For Like Growth Factor Binding Protein 1 (IGFBP1) Organism Species: Homo sapiens (Human) Instruction manual FOR RESEARCH USE ONLY NOT FOR USE
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01
Gather all necessary materials including the ELISA kit, samples, buffers, and reagents.
02
Prepare the sample by diluting it according to the kit instructions.
03
Add the diluted sample to the wells of the ELISA plate.
04
Incubate the plate at the specified temperature for the required time to allow the antigen to bind.
05
Wash the wells multiple times to remove unbound substances.
06
Add the detection antibody to each well and incubate again as per the kit instructions.
07
Wash the plate again to remove excess detection antibody.
08
Add the substrate solution to each well, which will react with the enzyme linked to the detection antibody.
09
Allow the reaction to develop for the time indicated in the instructions.
10
Stop the reaction by adding the stop solution provided.
11
Measure the optical density of each well using a microplate reader.
12
Analyze the results by comparing the readings to the standard curve provided with the kit.
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