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This manual provides detailed instructions for the use of the CircuLex Human REG4 ELISA Kit, which is designed for the quantitative measurement of human REG4 in research samples, including serum and plasma. It includes guidance on preparation, assay procedures, precautions, and troubleshooting methods.
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01
Gather all necessary materials, including the Circulex Human Reg4 ELISA kit, samples, and required diluents.
02
Prepare the sample dilutions as per the kit instructions, ensuring you have the correct concentration.
03
Add the samples or standards to the designated wells on the ELISA plate.
04
Incubate the plate as specified in the instructions, ensuring appropriate conditions (time and temperature).
05
Wash the wells multiple times with the wash buffer to remove unbound substances.
06
Add the detection antibody to each well and incubate again as per the kit's protocol.
07
Wash the wells again to remove excess antibody.
08
Add the substrate solution to each well and develop the color, monitoring the reaction time as specified.
09
Stop the reaction by adding the stop solution and mix gently.
10
Measure the optical density using a microplate reader at the specified wavelength.

Who needs circulex human reg4 elisa?

01
Researchers studying gastrointestinal diseases.
02
Clinics and hospitals seeking to assess regulatory T cell activity.
03
Pharmaceutical companies involved in drug development related to immune modulation.
04
Laboratories conducting biomarker screenings in various microbiome studies.
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Circulex Human Reg4 ELISA is an enzyme-linked immunosorbent assay (ELISA) kit designed to detect and quantify the levels of Reg4 protein in human samples for research purposes.
Researchers and laboratories conducting studies that require the measurement of Reg4 levels in human samples typically need to file information related to the use of Circulex Human Reg4 ELISA.
To fill out the Circulex Human Reg4 ELISA, users must follow the protocol provided in the kit, including preparing samples, conducting the assay steps, and recording the results according to the manufacturer's instructions.
The purpose of Circulex Human Reg4 ELISA is to provide a reliable method for measuring Reg4 protein levels, which can aid in understanding various diseases and biological processes.
The reported information typically includes sample preparation details, assay results, and any relevant controls or benchmarks used during the testing.
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