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bioRxiv preprint doi: https://doi.org/10.1101/2023.01.15.524171; this version posted January 18, 2023. The copyright holder for this preprint (which was not certified by peer review) is the author/funder,

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Begin by gathering the necessary information about the residues you will be assessing.

02

Open the form designated for single-residue effects.

03

For each residue, fill in the specific identification number or name in the designated field.

04

Input the relevant data for each residue, including its impact score or effect type, in the appropriate sections.

05

If applicable, note any specific conditions or mutations related to each residue.

06

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01

Researchers studying protein dynamics.

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What is single-residue effects on form?

Single-residue effects on form refer to the impact that the substitution of a single amino acid in a protein can have on its structure, function, or stability.

Who is required to file single-residue effects on form?

Researchers and organizations involved in protein engineering, biotechnology, and related fields are typically required to file single-residue effects on the form as part of regulatory compliance.

How to fill out single-residue effects on form?

To fill out the single-residue effects on form, one should provide details about the protein, specify the amino acid substitutions, and report the observed effects on its properties.

What is the purpose of single-residue effects on form?

The purpose of the single-residue effects on form is to ensure transparency in the evaluation of changes to proteins that may affect their safety, efficacy, or regulatory status.

What information must be reported on single-residue effects on form?

Information that must be reported includes the identity of the protein, the specific amino acid change, the rationale for the change, and any experimental data reflecting the impact of this change.

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