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Phage susceptibility testing and infectious titer determination through widefield lensless monitoring of phage plaque growth Prisca Perlemoine, Pierre R. Marcoux, Emmanuel Picard, Emmanuel Hadji,

How to fill out phage susceptibility testing and

How to fill out phage susceptibility testing and

1. Gather necessary materials: phage solutions, bacterial strains, agar plates, sterile pipettes, and incubation equipment.
2. Prepare agar plates by pouring molten agar mixed with the bacterial culture into sterile plates and letting it solidify.
3. Dilute the phage solutions to desired concentrations in sterile saline or broth.
4. Using a sterile pipette, add drops of the diluted phage solutions onto the prepared agar plates.
5. Incubate the plates at an appropriate temperature for the bacterial strain, typically 37°C for 18-24 hours.
6. After incubation, observe the plates for zones of inhibition surrounding the phage spots, indicating bacteriolytic activity.
7. Measure the diameter of the zones of inhibition to determine phage susceptibility.

Who needs phage susceptibility testing and?

1. Laboratories conducting microbiological research.
2. Clinical microbiologists to guide treatment options for bacterial infections.
3. Pharmaceutical companies developing phage therapy products.
4. Veterinary practitioners for animal health assessments.
5. Researchers studying bacterial resistance mechanisms.

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What is phage susceptibility testing?

Phage susceptibility testing is a laboratory method used to determine whether specific bacteria are susceptible or resistant to certain bacteriophages (viruses that infect bacteria). This testing is essential for the development of phage therapy as a treatment for bacterial infections.

Who is required to file phage susceptibility testing?

Entities involved in the research, development, and application of phage therapy, such as pharmaceutical companies, research institutions, and healthcare providers, may be required to file phage susceptibility testing results with regulatory authorities.

How to fill out phage susceptibility testing?

To fill out phage susceptibility testing, one must collect bacterial isolates, conduct a phage susceptibility assay, record the results, and compile the data in a standardized reporting format as prescribed by regulatory guidelines before submission.

What is the purpose of phage susceptibility testing?

The purpose of phage susceptibility testing is to evaluate the effectiveness of specific phages against targeted bacterial strains, ensuring that the chosen phages will effectively combat the infection in therapeutic applications.

What information must be reported on phage susceptibility testing?

The report on phage susceptibility testing must include details such as the bacterial strain tested, phage preparations used, testing methodology, results (including susceptibility or resistance), and any relevant controls.