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bioRxiv preprint doi: https://doi.org/10.1101/2024.06.09.598120; this version posted June 9, 2024. The copyright holder for this preprint (which was not certified by peer review) is the author/funder.
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01
Identify the relevant tissues or cells that will be analyzed for hypoxia and Sonic Hedgehog (Shh) signaling.
02
Measure oxygen levels in the tissue using appropriate sensors or assays to determine hypoxic conditions.
03
Assess the expression levels of Sonic Hedgehog ligands and receptors using techniques such as RT-PCR or Western blotting.
04
Analyze the effects of hypoxia on Sonic Hedgehog signaling pathways by conducting experiments with hypoxia mimetics or oxygen deprivation.
05
Collect and interpret data, correlating hypoxic conditions with changes in Shh expression and signaling activity.

Who needs hypoxia-sonic hedgehog axis as?

01
Researchers studying tumor microenvironments and cancer biology.
02
Clinicians investigating the role of oxygen levels in tissue regeneration.
03
Biologists exploring developmental processes and stem cell differentiation.
04
Pharmaceutical companies developing hypoxia-targeted therapies.
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The hypoxia-sonic hedgehog axis refers to the molecular signaling pathway that connects hypoxic conditions (low oxygen levels) to the activation of the Sonic Hedgehog (Shh) signaling pathway, which plays a crucial role in development and cellular differentiation.
Researchers and institutions conducting studies related to hypoxia and the Sonic Hedgehog pathway may be required to file reports or documentation concerning their findings and methodologies.
To fill out the documentation for the hypoxia-sonic hedgehog axis, one should gather relevant data from experiments, including methodologies, results, and interpretations related to hypoxic conditions and Sonic Hedgehog signaling, and follow the specified format of the submission guidelines.
The purpose of the hypoxia-sonic hedgehog axis is to understand how low oxygen conditions influence cellular functions and developmental processes through the Sonic Hedgehog signaling pathway, which has implications in various biological and medical fields.
The information that must be reported includes experimental setups, conditions of hypoxia, measurements taken, interpretations of results, and any significant findings related to the interactions between hypoxia and the Sonic Hedgehog pathway.
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