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PhytoFrontiersTM | 2023 r 3:148155https://doi.org/10.1094/PHYTOFR09220091FIResearch HighSensitivity ITS RealTime PCR Assays for Detection of Ceratocystis lukuohia and Ceratocystis huliohia in Soil
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01
Prepare your samples and reagents in a clean environment to avoid contamination.
02
Set up your high-sensitivity real-time PCR instrument as per the manufacturer's instructions.
03
Choose appropriate primers and probes specific to the target DNA or RNA you wish to detect.
04
Dilute your samples to the recommended concentration for optimal results.
05
Prepare a master mix by combining the polymerase, buffer, dNTPs, primers, and probes in one reaction tube.
06
Add the master mix to each reaction tube or well of the PCR plate.
07
Carefully add your prepared samples to each tube, mixing gently without introducing bubbles.
08
Seal the plates or tubes to prevent evaporation during the PCR process.
09
Load the reaction plate or tubes into the real-time PCR machine.
10
Select the appropriate PCR program and analyze the amplification data at the end of the run.
Who needs high-sensitivity its real-time pcr?
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Clinical laboratories for the detection of infectious diseases.
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Research institutions studying gene expression and mutation.
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Pharmaceutical companies conducting drug development and testing.
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Environmental agencies testing for pathogens or contaminants in samples.
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Agricultural sectors for testing plant diseases or genetically modified organisms.
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What is high-sensitivity its real-time pcr?
High-sensitivity ITS real-time PCR is a molecular diagnostic technique used to detect and quantify specific nucleic acid sequences in real-time, offering enhanced sensitivity compared to traditional PCR.
Who is required to file high-sensitivity its real-time pcr?
Entities that conduct testing for specific pathogens or genetic markers are required to file high-sensitivity ITS real-time PCR results.
How to fill out high-sensitivity its real-time pcr?
To fill out high-sensitivity ITS real-time PCR, follow the standardized reporting guidelines, ensuring accurate data entry of test results, sample information, and any relevant patient identification.
What is the purpose of high-sensitivity its real-time pcr?
The purpose of high-sensitivity ITS real-time PCR is to provide rapid and accurate detection of target genetic material, which aids in diagnosis and treatment decisions.
What information must be reported on high-sensitivity its real-time pcr?
Required information includes test results, sample identification, patient demographics, assay details, and any relevant clinical information.
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