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4D.Request for Council Action TO: THROUGH: FROM: MEETING DATE: SUBJECT:Mayor and City Council Tim Murray, City Administrator Mark DuChene, City Engineer September 11, 2018 Resolution 2018177 Declare
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How to fill out sample preparation in metabolomics

01
Collect samples: Obtain biological samples such as blood, urine, or tissue from the subjects of interest.
02
Stabilize samples: Use appropriate stabilization techniques (e.g., freezing, adding preservatives) to prevent metabolic changes.
03
Homogenize samples: Use a homogenizer or similar equipment to ensure the samples are uniform in texture.
04
Extract metabolites: Use solvent extraction techniques (e.g., organic solvents, water) to isolate metabolites from the sample matrix.
05
Clean-up: Perform purification steps (e.g., filtration, centrifugation) to remove proteins and other impurities from the extract.
06
Concentrate samples: If necessary, concentrate the extracted metabolites through evaporation or solid-phase extraction.
07
Prepare for analysis: Dilute and adjust the samples to the appropriate concentrations for specific analytical techniques, such as mass spectrometry or NMR.

Who needs sample preparation in metabolomics?

01
Researchers studying metabolic diseases to understand biochemical pathways.
02
Pharmaceutical companies developing new drugs that target metabolic processes.
03
Academic institutions conducting basic research in biochemistry and metabolomics.
04
Clinical laboratories performing diagnostic testing related to metabolic disorders.
05
Food and nutrition scientists assessing the metabolic effects of dietary components.
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Sample preparation in metabolomics refers to the process of preparing biological samples for analysis by various analytical techniques. This may involve steps such as extraction, purification, and concentration of metabolites to ensure accurate and reproducible results.
Researchers, laboratories, and institutions conducting metabolomics studies are required to file sample preparation methods and protocols as part of their compliance with regulatory and best practice guidelines.
To fill out sample preparation in metabolomics, one should detail the methods used for sample collection, handling, extraction, and any analytical techniques applied. This should include specific reagents, conditions, and protocols followed.
The purpose of sample preparation in metabolomics is to isolate and concentrate metabolites of interest, remove contaminants, and minimize variability in order to enhance the reliability and validity of analytical results.
Information that must be reported includes the source of the sample, details of the preparation process, reagents used, quantities, conditions (such as temperature and time), and any observed variables that may affect outcomes.
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