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This thesis investigates the production, optimization, and partial purification of extracellular lipase from Burkholderia cenocepacia ST8 through submerged fermentation utilizing used engine oil (UEO) as a substrate. The research addresses environmental pollution caused by improper UEO disposal and explores the potential of bioconverting UEO into commercially valuable lipase, focusing on optimizing fermentation conditions and employing aqueous two-phase systems for enzyme purification.

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